Dipeptide nanostructures: Synthesis, interactions, advantages and biomedical applications.

Short peptides are important in the design of self-assembled materials due to their versatility and flexibility. Self-assembled dipeptides, a group of peptide nanostructures, have highly attractive uses in the field of biomedicine. Recently these materials have proved to be important nanostructures because of their biocompatibility, low-cost and simplicity of synthesis, functionality/easy tunability and nano dimensions. Although there are different studies on peptide and protein-based nanostructures, more information about self-assembled nanostructures for dipeptides is still required to discover the advantages, challenges, importance, synthesis, interactions, and applications. This review describes and discusses the self-assembled dipeptide nanostructures especially for biomedical applications.

Comparison of microcomputed tomography, cone beam tomography, stereomicroscopy, and scanning electron microscopy techniques for detection of microcracks on root dentin and effect of different apical sizes on microcrack formation.

The aim of the study was to compare different imaging methods in the diagnosis of microcracks on root dentin and to evaluate the frequency of dentinal microcracks observed after root canal preparation using the ProTaper Universal (PTU) system of different sizes. A total of 30 mandibular molars' mesial roots were scanned with microcomputed tomography (micro-CT) and cone beam computed tomography (CBCT) imaging methods before instrumentation. Root canal instrumentation was performed up to PTU F2 and F4 files. After instrumentation stages, the roots were scanned again with micro-CT and then with CBCT in same parameters. All roots were sectioned horizontally at 2, 4, 6, 8, and 10 mm from the apices of the specimens. The sections were imaged under a stereomicroscope. Finally, imaging of the sections was done by scanning electron microscopy (SEM). Statistical data analysis of instrumentation steps was performed using Friedman and Wilcoxon tests, and the data of imaging methods were analyzed using Kruskal-Wallis and Mann-Whitney U tests (p = .05). Instrumentation with the PTU system up to F2 and F4 files significantly increased the number of microcracks compared with preoperative samples according to micro-CT imaging (p < .05). For detecting microcracks on the root dentin, there were no statistically significant differences between micro-CT (43.9%) and stereomicroscopy (45.8%) (p < .05). SEM showed significantly higher percentage of microcracks (88.3%) (p > .05). No microcrack was observed using the CBCT method. There were no statistically differences between micro-CT and stereomicroscopy. SEM showed more dentinal microcracks while no microcrack was observed with CBCT.

Evaluation of Different Techniques for Filling Simulated C-shaped Canals of 3-dimensional Printed Resin Teeth.

INTRODUCTION: The purpose of this study was to compare the ability of different root canal filling methods on the C1 root canal anatomy using 3-dimensional (3D) printer technology. METHODS: Mandibular molars were scanned with cone-beam computed tomographic (CBCT) imaging. A mandibular molar tooth with a C1 root canal configuration was selected according to images obtained from CBCT technology. The root canals were shaped with ProTaper Universal rotary instruments (Dentsply Maillefer, Ballaigues, Switzerland) and hand files. After the root canal shaping procedure, the tooth was scanned with micro-computed tomographic imaging, and 80 replicas of the tooth were produced with a 3D printer. Replicas were assigned into 4 groups (n = 20) and were filled using one of the following techniques: a core carrier system (GuttaCore [GC]; Dentsply Tulsa Dental Specialties, Tulsa, OK), a continuous wave obturation system (Elements Free [CW]; Kerr/SybronEndo, Orange, CA), a cold lateral compaction technique (CLC), or the injectable cold filling method (GuttaFlow Bioseal [GFB]; Coltène/Whaledent AG, Altstatten, Switzerland). All specimens were sectioned horizontally at 2, 4, 6, 8, and 10 mm from the apices of the specimens. The sections were examined at 32× magnification under a stereomicroscope, and the percentage areas of gutta-percha, sealer, and voids were measured. Statistical analysis of data was performed using multivariate analysis of variance and Tukey honestly significant difference tests (P < .05). RESULTS: The CW group had the highest mean percentages for void and the least mean percentages for gutta-percha at 2-mm sections (P < .05). A pairwise comparison between the groups showed significantly less void in the CLC and GFB groups and more void in the CW and GC groups at 2 mm (P < .05). In the total area, the CLC and GFB groups showed a significantly lower percentage of gutta-percha and more sealer compared with the other groups (P < .05), and there was no significant difference between the CLC and GFB groups (P > .05). CONCLUSIONS: At the apical part of the canal, the cold lateral compaction and cold injectable filling techniques were more successful compared with the warm gutta-percha filling techniques in C1 root canal configuration.

Fracture resistance of simulated immature teeth after apexification with calcium silicate-based materials.

OBJECTIVE: To compare the fracture resistance of simulated immature teeth filled with an apical barrier of mineral trioxide aggregate (MTA), Biodentine, and calcium-enriched mixture (CEM). MATERIALS AND METHODS: Fifty-two single-rooted human maxillary central incisors were used. For standardization, the teeth were sectioned 6 mm above and 9 mm below the cementoenamel junction to simulate immature apex. Simulations of roots into immature apices were carried out using 1.5 mm diameter drills. The specimens were then randomly divided into three experimental groups (n = 13) and one control group (n = 13). In experimental groups, MTA, Biodentine, and CEM were placed to apical 4 mm of the simulated immature roots. The samples were stored at 37° C and 100% humidity for 1 week. A load was applied on the crown of all teeth at 135° to their long axis until fracture. The data were analyzed using one-way analysis of variance and Tukey post-hoc tests. RESULTS: No statistically significant differences were found among MTA, CEM, and Biodentine (P > 0.05), and these groups demonstrated higher fracture resistance than control group (P < 0.05). CONCLUSIONS: Using any of the MTA, Biodentine, and CEM as an apical plug and restoring with fiber post and composite resin increases the fracture resistance of immature teeth.

Comparison of retreatment ability of full-sequence reciprocating instrumentation and 360° rotary instrumentation.

OBJECTIVES: The purpose of the present study was to investigate the amount of root canal filling material after root canal filling removal with 360° rotary instrumentation or reciprocating motion with the same file sequence. MATERIALS AND METHODS: Root canals of the 36 mandibular premolars were shaped with ProTaper Universal instruments up to size F2 and filled with corresponding single gutta-percha cone and sealer. The teeth were assigned to two retreatment groups (n = 18): group 1 360° rotational motion and group 2 reciprocating motion of ATR Tecnika motors (1310° clockwise and 578° counterclockwise). Retreatment procedure was performed with ProTaper Universal retreatment files with a sequence of D1-3 and ProTaper Universal F3 instruments. Total time required to remove filling material were recorded. Remaining filling material was examined under stereomicroscope at ×8 magnification. The data were analysed statistically using the Mann-Whitney U test, and testing was performed at 95 % confidence level (p < 0.05). RESULTS: There were no significant differences between the groups (p > 0.05) in terms of remaining filling material. The total time required for retreatment was shorter in 360° rotational motion group compared to reciprocating motion group (p < 0.05). CONCLUSION: Both continuous rotation and reciprocating motion showed similar effectiveness in terms of root canal filling material removal. CLINICAL RELEVANCE: Using ProTaper Universal retreatment instruments with reciprocating motion of ATR motor and conventional rotary motion have similar efficacy in root canal filling removal.

Effects of root canal preparation, various filling techniques and retreatment after filling on vertical root fracture and crack formation.

AIM: To investigate the incidence of cracks in root dentin after rotary instrumentation and filling with cold lateral condensation (CL), single-cone obturation (SC), and warm vertical compaction (WV) and retreatment of each filling system. METHODOLOGY: One-hundred sixty mandibular incisors were selected. Twenty teeth were left unprepared and served as a control, and the remaining 140 teeth were instrumented. Twenty teeth were assigned to an only prepared group. The remaining 120 teeth were assigned to three root canal filling groups and three retreatment groups (n = 20), as follows: CL, WV, SC, and retreatment of each filling system. All the roots were sectioned perpendicular to the long axis at 2, 4, 6, and 8 mm from the apex, and the sections were then observed under a stereomicroscope. The absence/presence of cracks and vertical root fractures were recorded, and the data were analyzed with a Pearson's chi-square test (P = 0.05). RESULTS: No complete vertical root fractures were observed in the unprepared and only prepared groups. However, vertical fractures were observed in the filling and retreatment groups. There were significantly more cracks in the retreatment after WV group than the other groups, with the exception of the retreatment after SC, WV, and CL resulted in significantly more cracks than the only prepared and SC. CONCLUSIONS: The filling techniques (except SC) caused more cracks than preparation only. The SC caused fewer cracks compared to the other filling techniques. The retreatment following the different filling techniques (except CL) caused more cracks than the original fillings.

Evaluation of effectiveness of various irrigating solutions on removal of calcium hydroxide mixed with 2% chlorhexidine gel and detection of orange-brown precipitate after removal.

INTRODUCTION: The aims of the present study were to evaluate the effect of various irrigating solutions on the removal of calcium hydroxide mixed with 2% chlorhexidine gel from an artificial groove created in a root canal and the generation of orange-brown precipitate in the remaining calcium hydroxide mixed with 2% chlorhexidine gel after irrigation with the various irrigating solutions. METHODS: The root canals of 48 mandibular premolars were prepared using ProTaper Universal Rotary instruments (Dentsply Maillefer, Ballaigues, Switzerland) up to size F4. The roots were split longitudinally, and a standardized groove was prepared in the apical part of 1 segment. The root halves were reassembled, and calcium hydroxide mixed with 2% chlorhexidine gel medicament was placed into the grooves. The roots were randomly divided into 4 experimental groups specified by the irrigation solution used: 1% NaOCl, 17% EDTA, 7% maleic acid, and 10% citric acid (n = 12). The amount of remaining medicament was evaluated under a stereomicroscope using a 4-grade scoring system. After irrigation, the specimens were also evaluated for the presence/absence of orange-brown precipitate. The effects of the different irrigation solutions on medicament removal were statistically evaluated using the Kruskal-Wallis and Mann-Whitney U tests with Bonferroni correction at a 95% confidence level (P = .0083). RESULTS: Solutions of 7% maleic acid and 10% citric acid were superior to solutions of 1% NaOCl and 17% EDTA in removing calcium hydroxide mixed with 2% chlorhexidine gel (P < .0083). There were no significant differences among the other groups (P > .0083). Orange-brown precipitate was observed in all specimens of the NaOCl group but in no specimens in the other groups. CONCLUSIONS: Irrigation solutions of 7% maleic acid and 10% citric acid were more effective in the removal of calcium hydroxide mixed with 2% chlorhexidine gel than those of 1% NaOCl and 17% EDTA. Orange-brown precipitate was found in all specimens of the NaOCl-irrigated groups. However, the precipitate was not observed in specimens in the groups irrigated with 17% EDTA, 7% maleic acid, and 10% citric acid.

Effect of photo-activated disinfection on bond strength of three different root canal sealers.

INTRODUCTION: The aim of this in vitro study was to evaluate the bond strength of Photo-Activated Disinfection (PAD) system to dentin with different root canal sealers by using a push-out test design. MATERIALS AND METHODS: A total of 30 extracted mandibular premolar teeth with single and straight roots were used. The crowns were removed and the root canals were prepared by using ProTaper rotary files. The smear layer was removed and the roots were randomly divided into two groups (n = 15) according to the use of PAD system as the final disinfecting agent. Each group was then divided into 6 (n = 5) subgroups and obturated with gutta-percha and 3 different root canal sealers. The groups were Group 1: Sodium hypochlorite (NaOCl) + ethylenediaminetetraacetic acid (EDTA)-AH Plus sealer; Group 2: NaOCl + EDTA + PAD-AH Plus; Group 3: NaOCl + EDTA-Sealapex; Group 4: NaOCl + EDTA + PAD-Sealapex; Group 5: NaOCl + EDTA-mineral trioxide aggregate (MTA)-Fiallapex; and Group 6: NaOCl + EDTA + PAD-MTA-Fillapex. 1-mm thickness horizontal sections (n: 5 × 4 = 20) were sliced for the push-out bond strength measurement. RESULTS: Group 3 and 4 showed significantly lower bond strengths compared with all the other groups (P < 0.05). No statistically significant difference was found among Groups 1, 2 and 5, but there was statistically significant difference between Group 5 and 6 (P < 0.05). CONCLUSION: This in vitro study indicated that the PAD system adversely affected the bond strength of the MTA Fillapex root canal sealer.

Effect of photoactivated disinfection on bond strength of root canal filling.

INTRODUCTION: The aim of this in vitro study was to assess and compare the effect of photoactivated disinfection (PAD) on the bond strength of root canal sealers to human root canal dentin using the push-out test. METHODS: Fifteen extracted human mandibular premolar teeth with single and straight roots were used. After the clinical crowns were removed from the cementoenamel junction, root canals were prepared with the ProTaper (Dentsply Maillefer, Ballaigues, Switzerland) rotary system to the size of the F3 file. The smear layer of the roots was removed using 17% EDTA followed by 5.25% sodium hypochlorite (NaOCl) and distillate water. The roots were then randomly divided into 3 groups (n = 5) according to the final irrigation regimen. In group 1, PAD (FotoSan; CMS Dental, Copenhagen, Denmark) was applied to the root canals and light cured for 20 seconds. Group 2 was finally irrigated with a 2% solution of chlorhexidine gluconate, and group 3 served as the control group (NaOCl + EDTA). All the canals were then obturated with the lateral condensation technique using gutta-percha and AH Plus sealer (Dentsply Maillefer) sealer. One-millimeter-thick horizontal sections from the coronal and midthirds of each root (n: 5 × 4 = 20) were sliced for the push-out bond strength measurement. The data were converted to megapascals and statistically analyzed using 1-way analysis of variance and the post hoc Tukey test. RESULTS: There was no significant difference among the bond strength of PAD, chlorhexidine gluconate, and NaOCl (P > .05). CONCLUSIONS: We conclude that PAD does not adversely affect the bond strength of the AH Plus sealer to root canal dentin and that it can be used for the final disinfection of root canals.